Berkeley Lab scientist Ee-Been Goh thrives on re-engineering bacteria and mentoring students.
To see biochemist Ee-Been Goh in the lab today, figuring out how to rewire bacteria to produce biofuels, one would never guess she was once so uninterested in school that she barely made it through junior high. (more…)
New Technique Identifies Populations Within a Microbial Community Responsible for Biomass Deconstruction
One of the keys to commercialization of advanced biofuels is the development of cost-competitive ways to extract fermentable sugars from lignocellulosic biomass. The use of enzymes from thermophiles – microbes that thrive at extremely high temperatures and alkaline conditions – holds promise for achieving this. Finding the most effective of these microbial enzymes, however, has been a challenge. That challenge has now been met by a collaboration led by researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI).
Working with a compost-derived consortium of thermophillic bacterium adapted to grow on switchgrass, a leading potential fuel crop, and using a combination of metagenomic and metaproteomic technologies, the collaboration has identified individual microbial species whose enzymes were the most active in deconstructing the switchgrass biomass. Major institutes in addition to JBEI participating in this collaboration included DOE’s Joint Genome Institute (JGI), and EMSL, the Environmental Molecular Sciences Laboratory, a national scientific user facility at Pacific Northwest National Laboratory (PNNL). (more…)
Enzymes evolved in the lab hold commercial and scientific promise
Whether big, small, slimy, or tall, most animal bodies are symmetric.
Except for sea anemones, starfish, sponges, and the like, animals have bilateral, or right-left, symmetry. Us included.
The bilateral body plan became the norm over eons of evolution. But what about molecules? Have any evolved common structures like a body plan? (more…)
Researchers Show Ionic Liquids Effective for Pre-Treating Mixed Blends of Biofuel Feedstocks
Winemakers have long known that blending different grape varietals can favorably balance the flavor characteristics of the wine they produce. In the future, makers of advanced biofuels might use a similar strategy, blending different feedstock varieties to balance the energy characteristics of the transportation fuel they produce.
A collaborative study by researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI), a bioenergy research center led by Berkeley Lab, and the Idaho National Laboratory (INL) has shown that an ionic liquid proven to be effective for pre-treating individual biofuel feedstocks is also effective at pre-treating multiple different feedstocks that have been mixed and densified into a blend. (more…)
Collaboration at JBEI Identifies the First Enzyme Linked to Galactan Synthesis
Galactan is a polymer of galactose, a six-carbon sugar that can be readily fermented by yeast into ethanol and is a target of interest for researchers in advanced biofuels produced from cellulosic biomass. Now an international collaboration led by scientists at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) has identified the first enzyme capable of substantially boosting the amount of galactan in plant cell walls.
Unlike ethanol, advanced biofuels synthesized from the sugars in plant cells walls could replace gasoline, diesel and jet fuels on a gallon-for-gallon basis and be dropped into today’s engines and infrastructures with no modifications required. Also, adanced biofuels have the potential to be carbon-neutral, meaning they could be burned without adding excess carbon to the atmosphere. Among the key challenges to making advanced biofuels cost competitive is finding ways to maximize the amount of plant cell wall sugars that can be fermented into fuels. (more…)
Berkeley Lab Researchers Combine Old Fermentation Process For Making Explosives with New Chemical Catalysis to Boost Biofuel Production
A fermentation technique once used to make cordite, the explosive propellant that replaced gunpowder in bullets and artillery shells, may find an important new use in the production of advanced biofuels. With the addition of a metal catalyst, researchers at the U.S. Department of Energy (DOE)’s Lawrence Berkeley National Laboratory (Berkeley Lab) have shown that the production of acetone, butanol and ethanol from lignocellulosic biomass could be selectively upgraded to the high volume production of gasoline, diesel or jet fuel.
Using the bacterium Clostridium acetobutylicum, the Berkeley Lab researchers fermented the sugars found in biomass into the solvent acetone and the alcohols butanol and ethanol, collectively known as “ABE” products. They then catalyzed these low carbon number products with the transition metal palladium into higher-molecular-mass hydrocarbons that are possible precursors to the three major transportation fuel molecules. The specific type of fuel molecule produced – whether a precursor to gasoline, diesel or jet – was determined by the amount of time the ABE products resided with the palladium catalyst. (more…)
TeselaGen’s DNA construction technology makes genetic engineering cheaper and faster.
Sequencing, splicing and expressing DNA may seem to be the quintessence of cutting-edge science—indeed DNA manipulation has revolutionized fields such as biofuels, chemicals and medicine. But in fact, the actual process can still be tedious and labor-intensive, something Lawrence Berkeley National Laboratory (Berkeley Lab) scientist Nathan Hillson learned the hard way.
After struggling for two days to design a protocol to put together a genetic circuit with 10 pieces of DNA—using a spreadsheet as his primary tool—he was dismayed to discover that an outside company could have done the whole thing, including parts and labor, for lower cost than him ordering the oligonucleotides himself. “I learned two things: one, I never wanted to go through that process again, and two, it’s extremely important to do the cost-effectiveness calculation,” said Hillson, a biochemist who also directs the synthetic biology program at the Berkeley Lab-led Joint BioEnergy Institute (JBEI). “So that was the genesis of the j5 software. This is the perfect thing to teach a computer to do.” (more…)
Scientists find key protein for algae growth in the ocean
Scientists have revealed a key cog in the biochemical machinery that allows marine algae at the base of the oceanic food chain to thrive. They have discovered a previously unknown protein in algae that grabs an essential but scarce nutrient out of seawater, vitamin B12.
Many algae, as well as land-dwelling animals, including humans, require B12, but they cannot make it and must either acquire it from the environment or eat food that contains B12. Only certain single-celled bacteria and archaea have the ability to synthesize B12, which is also known as cobalamin. (more…)
