Tag Archives: cellulosic biomass

Microbial Who-Done-It for Biofuels

By Guest PostAugust 18, 2013Scienceadvanced biofuels, alkaline conditions, biofuels, blake simmons, cellulosic biomass, commercialization, diesel, gasoline, ionic liquids, jbei, jet fuels, john gladden, lignin, lignocellulosic biomass, martin allgaier, metagenomic, metaproteomic technologies, microbes, microbial enzymes, microbial populations, patrik chain, patrik dhaeseleer, renewable alternative, rhodothermus marinus, steven singer, switchgrass, thermophiles, thermophillic bacterium, thermus thermophilus, use of enzymes, woody material

New Technique Identifies Populations Within a Microbial Community Responsible for Biomass Deconstruction

One of the keys to commercialization of advanced biofuels is the development of cost-competitive ways to extract fermentable sugars from lignocellulosic biomass. The use of enzymes from thermophiles – microbes that thrive at extremely high temperatures and alkaline conditions – holds promise for achieving this. Finding the most effective of these microbial enzymes, however, has been a challenge. That challenge has now been met by a collaboration led by researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI).

Working with a compost-derived consortium of thermophillic bacterium adapted to grow on switchgrass, a leading potential fuel crop, and using a combination of metagenomic and metaproteomic technologies, the collaboration has identified individual microbial species whose enzymes were the most active in deconstructing the switchgrass biomass. Major institutes in addition to JBEI participating in this collaboration included DOE’s Joint Genome Institute (JGI), and EMSL, the Environmental Molecular Sciences Laboratory, a national scientific user facility at Pacific Northwest National Laboratory (PNNL). (more…)

New Advance in Biofuel Production

By Guest PostJune 2, 2013Environmentacid catalyst, advanced biofuels, alternative to gasoline, atmosphere, bioenergy, biofuel production, blake simmons, burning, carbon dioxide, cellulosic biomass, crystals, diesel, enzymes, fermentable sugars, fossil fuels, hanbin liu noppadon, hydrolyzing biomass polysaccharides, imidazolium chloride, ionic liquid, jbei, jet fuels, liquid fuels, manali sawant, new advance, ning sun, recycle, vitalie stavila

Joint BioEnergy Institute Researchers Develop Enzyme-free Ionic Liquid Pre-treatment

Advanced biofuels – liquid fuels synthesized from the sugars in cellulosic biomass – offer a clean, green and renewable alternative to gasoline, diesel and jet fuels. Bringing the costs of producing these advanced biofuels down to competitive levels with petrofuels, however, is a major challenge. Researchers at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI), a bioenergy research center led by Berkeley Lab, have taken another step towards meeting this challenge with the development of a new technique for pre-treating cellulosic biomass with ionic liquids – salts that are liquids rather than crystals at room temperature. This new technique requires none of the expensive enzymes used in previous ionic liquid pretreatments, and makes it easier to recover fuel sugars and recycle the ionic liquid. (more…)

Sweet Success: Berkeley Lab Researchers Find Way to Catalyze More Sugars from Biomass

By Guest PostMay 4, 2013Scienceachilles heel, advanced biofuels, amorphous, berkeley lab, biofuel production, biomass of grasses, carbon into atmosphere, catalysis, catalytic activity, cellulase enzymes, cellulose classification system, cellulosic biomass, crystalline, douglas clark, enzyme cocktails, enzyme synergy, fermentable sugars, fossil fuels, harvey blanch, industrial manufacturing processes, jan liphardt, jerome fox, light microscopy technique, localization microscopy, palm, palm technology, photo activated, solid surfaces, substrate heterogeneity, sweet success, synergistic activity, ultrahigh precision, united states, university of california

Catalysis may initiate almost all modern industrial manufacturing processes, but catalytic activity on solid surfaces is poorly understood. This is especially true for the cellulase enzymes used to release fermentable sugars from cellulosic biomass for the production of advanced biofuels. Now, researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) through support from the Energy Biosciences Institute (EBI) have literally shed new light on cellulase catalysis.

Using an ultrahigh-precision visible light microscopy technique called PALM – for Photo-Activated Localization Microscopy – the researchers have found a way to improve the collective catalytic activity of enzyme cocktails that can boost the yields of sugars for making fuels. Increasing the sugar yields from cellulosic biomass to help bring down biofuel production costs is essential for the widespread commercial adoption of these fuels. (more…)

Biofuels Blend Right In

By Guest PostFebruary 3, 2013Environmentadvanced biofuels industry, atmosphere warming carbon, balance, benchmark, berkeley lab, biofuel feedstocks, biofuels, blake simmons, cellulosic biomass, complex polysaccharides, corn stover, cost competitive, diesel, energy characteristics, energy densification, eucalyptus, feedstock varieties, fermentable sugars, flavor characteristics, fossil fuels, gasoline, global temperatures, grape varietals, green chemistry, ionic liquid, jbei, jet fuel, jian shi, joint bioenergy institute, lignin, lignocellulosic biomass, lignocellulosic biorefineries, lodgepole pine, methylimidazolium acetate, microbial fermentation, mixture, neal yancey, pellets, pre treatment, recalcitrant material, seema singh, substitute, switchgrass, Technology, transportation fuel, united states, vicki thompson, vitalie stavila, volatile organic solvents, wine, winemakers

Researchers Show Ionic Liquids Effective for Pre-Treating Mixed Blends of Biofuel Feedstocks

Winemakers have long known that blending different grape varietals can favorably balance the flavor characteristics of the wine they produce. In the future, makers of advanced biofuels might use a similar strategy, blending different feedstock varieties to balance the energy characteristics of the transportation fuel they produce.

A collaborative study by researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI), a bioenergy research center led by Berkeley Lab, and the Idaho National Laboratory (INL) has shown that an ionic liquid proven to be effective for pre-treating individual biofuel feedstocks is also effective at pre-treating multiple different feedstocks that have been mixed and densified into a blend. (more…)

Boosting Galactan Sugars Could Boost Biofuel Production

By Guest PostDecember 23, 2012Environmentai oikawa, april liwanag, arabidopsis thaliana, atmosphere, berit ebert, berkeley lab, bioenergy fuel crops, biofuels, carbon neutral, carsten rautengarten, cellulosic biomass, denmark, diesel, emilie rennie, enzyme, ethanol, ferment, fuels, galactan, galactan synthase, galactose, gals1, gals2, gals3, gasoline, gt92 enzyme, gt92 proteins, henrik scheller, jet fuels, mads clausen, mathias andersen, mechanical stress, miscanthus, overexpressors, pentoses, plant cell walls, polymer, six carbon sugar, snowfall, switchgrass, technical university, wind, yeast, yves verhertbruggen, β galactan synthase

Collaboration at JBEI Identifies the First Enzyme Linked to Galactan Synthesis

Galactan is a polymer of galactose, a six-carbon sugar that can be readily fermented by yeast into ethanol and is a target of interest for researchers in advanced biofuels produced from cellulosic biomass. Now an international collaboration led by scientists at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) has identified the first enzyme capable of substantially boosting the amount of galactan in plant cell walls.

Unlike ethanol, advanced biofuels synthesized from the sugars in plant cells walls could replace gasoline, diesel and jet fuels on a gallon-for-gallon basis and be dropped into today’s engines and infrastructures with no modifications required. Also, adanced biofuels have the potential to be carbon-neutral, meaning they could be burned without adding excess carbon to the atmosphere. Among the key challenges to making advanced biofuels cost competitive is finding ways to maximize the amount of plant cell wall sugars that can be fermented into fuels. (more…)

Microbe That Can Handle Ionic Liquids

By Guest PostMay 17, 2012Environment, Scienceanthropogenic chemicals, biofuel production, biofuels, blake simmons, carbon, cellulosic biomass, enterobacter lignolyticus, fossil fuels, hannah wooa, industry, ionic liquids, jane khudyakov, jbei, kristen deangelis, michael thelen, microbe, microorganisms, model, patrik dhaeseleer, properties, scf1 strain, semicrystalline, sharon borglin, soil bacterium, sugar fermenting microbes, switchgrass, Technology, toxic effect, tropical rainforest microbe

New Find From Joint BioEnergy Institute Could Help Reduce Biofuel Production Costs

In the search for technology by which economically competitive biofuels can be produced from cellulosic biomass, the combination of sugar-fermenting microbes and ionic liquid solvents looks to be a winner save for one major problem: the ionic liquids used to make cellulosic biomass more digestible for microbes can also be toxic to them. A solution to this conundrum, however, may be in the offing.

Researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI), a multi-institutional partnership led by Berkeley Lab, have identified a tropical rainforest microbe that can endure relatively high concentrations of an ionic liquid used to dissolve cellulosic biomass. The researchers have also determined how the microbe is able to do this, a discovery that holds broad implications beyond the production of advanced biofuels. (more…)

A Fragrant New Biofuel

By Guest PostMarch 16, 2012Environmentadvanced biofuel, agricultural waste, alkene hydrocarbon, bacteria, berkeley lab, biofuels, biofuels industry, cellulosic biomass, chemical compound, degree of unsaturation, diesel, e coli, edward baidoo, ee been goh, escherichia coli, fatty acid, flavor, fragrance, fuel molecule, fuel properties, gasoline, genetic modification, glucose, harry beller, jay keasling, jbei, jet fuel, lynn yarris, methyl ketone, microbial production, octane, perennial grasses, plant cell, renewable fuel, synthesis, tridecanone, undecanone

*JBEI Researchers Develop a New Candidate for a Cleaner, Greener and Renewable Diesel Fuel*

A class of chemical compounds best known today for fragrance and flavor may one day provide the clean, green and renewable fuel with which truck and auto drivers fill their tanks. Researchers at the U.S. Department of Energy’s Joint BioEnergy Institute (JBEI) have engineered Escherichia coli (E. coli) bacteria to generate significant quantities of methyl ketone compounds from glucose. In subsequent tests, these methyl ketones yielded high cetane numbers – a diesel fuel rating comparable to the octane number for gasoline – making them strong candidates for the production of advanced biofuels.

“Our findings add to the list of naturally occurring chemical compounds that could serve as biofuels, which means more flexibility and options for the biofuels industry,” says Harry Beller, a JBEI microbiologist who led this study. “We’re especially encouraged by our finding that it is possible to increase the methyl ketone titer production of E. coli more than 4,000-fold with a relatively small number of genetic modifications.” (more…)