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Microbial Who-Done-It for Biofuels

By Guest PostAugust 18, 2013Scienceadvanced biofuels, alkaline conditions, biofuels, blake simmons, cellulosic biomass, commercialization, diesel, gasoline, ionic liquids, jbei, jet fuels, john gladden, lignin, lignocellulosic biomass, martin allgaier, metagenomic, metaproteomic technologies, microbes, microbial enzymes, microbial populations, patrik chain, patrik dhaeseleer, renewable alternative, rhodothermus marinus, steven singer, switchgrass, thermophiles, thermophillic bacterium, thermus thermophilus, use of enzymes, woody material

New Technique Identifies Populations Within a Microbial Community Responsible for Biomass Deconstruction

One of the keys to commercialization of advanced biofuels is the development of cost-competitive ways to extract fermentable sugars from lignocellulosic biomass. The use of enzymes from thermophiles – microbes that thrive at extremely high temperatures and alkaline conditions – holds promise for achieving this. Finding the most effective of these microbial enzymes, however, has been a challenge. That challenge has now been met by a collaboration led by researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI).

Working with a compost-derived consortium of thermophillic bacterium adapted to grow on switchgrass, a leading potential fuel crop, and using a combination of metagenomic and metaproteomic technologies, the collaboration has identified individual microbial species whose enzymes were the most active in deconstructing the switchgrass biomass. Major institutes in addition to JBEI participating in this collaboration included DOE’s Joint Genome Institute (JGI), and EMSL, the Environmental Molecular Sciences Laboratory, a national scientific user facility at Pacific Northwest National Laboratory (PNNL). (more…)

Microbe That Can Handle Ionic Liquids

By Guest PostMay 17, 2012Environment, Scienceanthropogenic chemicals, biofuel production, biofuels, blake simmons, carbon, cellulosic biomass, enterobacter lignolyticus, fossil fuels, hannah wooa, industry, ionic liquids, jane khudyakov, jbei, kristen deangelis, michael thelen, microbe, microorganisms, model, patrik dhaeseleer, properties, scf1 strain, semicrystalline, sharon borglin, soil bacterium, sugar fermenting microbes, switchgrass, Technology, toxic effect, tropical rainforest microbe

New Find From Joint BioEnergy Institute Could Help Reduce Biofuel Production Costs

In the search for technology by which economically competitive biofuels can be produced from cellulosic biomass, the combination of sugar-fermenting microbes and ionic liquid solvents looks to be a winner save for one major problem: the ionic liquids used to make cellulosic biomass more digestible for microbes can also be toxic to them. A solution to this conundrum, however, may be in the offing.

Researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI), a multi-institutional partnership led by Berkeley Lab, have identified a tropical rainforest microbe that can endure relatively high concentrations of an ionic liquid used to dissolve cellulosic biomass. The researchers have also determined how the microbe is able to do this, a discovery that holds broad implications beyond the production of advanced biofuels. (more…)

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Microbial Who-Done-It for Biofuels

Microbe That Can Handle Ionic Liquids