Berkeley Lab scientist Ee-Been Goh thrives on re-engineering bacteria and mentoring students.
To see biochemist Ee-Been Goh in the lab today, figuring out how to rewire bacteria to produce biofuels, one would never guess she was once so uninterested in school that she barely made it through junior high.(more…)
Collaboration at JBEI Identifies the First Enzyme Linked to Galactan Synthesis
Galactan is a polymer of galactose, a six-carbon sugar that can be readily fermented by yeast into ethanol and is a target of interest for researchers in advanced biofuels produced from cellulosic biomass. Now an international collaboration led by scientists at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) has identified the first enzyme capable of substantially boosting the amount of galactan in plant cell walls.
Unlike ethanol, advanced biofuels synthesized from the sugars in plant cells walls could replace gasoline, diesel and jet fuels on a gallon-for-gallon basis and be dropped into today’s engines and infrastructures with no modifications required. Also, adanced biofuels have the potential to be carbon-neutral, meaning they could be burned without adding excess carbon to the atmosphere. Among the key challenges to making advanced biofuels cost competitive is finding ways to maximize the amount of plant cell wall sugars that can be fermented into fuels. (more…)
Berkeley Lab Researchers Combine Old Fermentation Process For Making Explosives with New Chemical Catalysis to Boost Biofuel Production
A fermentation technique once used to make cordite, the explosive propellant that replaced gunpowder in bullets and artillery shells, may find an important new use in the production of advanced biofuels. With the addition of a metal catalyst, researchers at the U.S. Department of Energy (DOE)’s Lawrence Berkeley National Laboratory (Berkeley Lab) have shown that the production of acetone, butanol and ethanol from lignocellulosic biomass could be selectively upgraded to the high volume production of gasoline, diesel or jet fuel.
Using the bacterium Clostridium acetobutylicum, the Berkeley Lab researchers fermented the sugars found in biomass into the solvent acetone and the alcohols butanol and ethanol, collectively known as “ABE” products. They then catalyzed these low carbon number products with the transition metal palladium into higher-molecular-mass hydrocarbons that are possible precursors to the three major transportation fuel molecules. The specific type of fuel molecule produced – whether a precursor to gasoline, diesel or jet – was determined by the amount of time the ABE products resided with the palladium catalyst. (more…)
TeselaGen’s DNA construction technology makes genetic engineering cheaper and faster.
Sequencing, splicing and expressing DNA may seem to be the quintessence of cutting-edge science—indeed DNA manipulation has revolutionized fields such as biofuels, chemicals and medicine. But in fact, the actual process can still be tedious and labor-intensive, something Lawrence Berkeley National Laboratory (Berkeley Lab) scientist Nathan Hillson learned the hard way.
After struggling for two days to design a protocol to put together a genetic circuit with 10 pieces of DNA—using a spreadsheet as his primary tool—he was dismayed to discover that an outside company could have done the whole thing, including parts and labor, for lower cost than him ordering the oligonucleotides himself. “I learned two things: one, I never wanted to go through that process again, and two, it’s extremely important to do the cost-effectiveness calculation,” said Hillson, a biochemist who also directs the synthetic biology program at the Berkeley Lab-led Joint BioEnergy Institute (JBEI). “So that was the genesis of the j5 software. This is the perfect thing to teach a computer to do.” (more…)
Scientists believe they’ve pinpointed the last crucial piece of the 80-year-old puzzle of how plants “know” when to flower.
Determining the proper time to flower, important if a plant is to reproduce successfully, involves a sequence of molecular events, a plant’s circadian clock and sunlight.
Understanding how flowering works in the simple plant used in this study – Arabidopsis – should lead to a better understanding of how the same genes work in more complex plants grown as crops such as rice, wheat and barley, according to Takato Imaizumi, a University of Washington assistant professor of biology and corresponding author of a paper in the May 25 issue of the journal Science. (more…)
New Find From Joint BioEnergy Institute Could Help Reduce Biofuel Production Costs
In the search for technology by which economically competitive biofuels can be produced from cellulosic biomass, the combination of sugar-fermenting microbes and ionic liquid solvents looks to be a winner save for one major problem: the ionic liquids used to make cellulosic biomass more digestible for microbes can also be toxic to them. A solution to this conundrum, however, may be in the offing.
Researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI), a multi-institutional partnership led by Berkeley Lab, have identified a tropical rainforest microbe that can endure relatively high concentrations of an ionic liquid used to dissolve cellulosic biomass. The researchers have also determined how the microbe is able to do this, a discovery that holds broad implications beyond the production of advanced biofuels. (more…)
*ARPA-E funded project aims to produce fuel molecules in plant leaves*
Mention biofuels and most people think of corn ethanol. Some may think of advanced biofuels from switchgrass or miscanthus. But tobacco? Not likely.
That could change. A team of scientists led by a researcher from the U.S. Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab) is exploring a way to produce gasoline, diesel, and jet fuel from the iconic plant of the South. (more…)