JBEI researchers use engineered bacteria to simplify biofuels production, potentially lowering cost
Researchers at the U.S. Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab) have engineered a strain of bacteria that enables a “one-pot” method for producing advanced biofuels from a slurry of pre-treated plant material. (more…)
Joint BioEnergy Institute Researchers Combine Systems Biology with Genetic Engineering to Improve Production of Isopentenol in E.Coli
In the on-going effort to develop advanced biofuels as a clean, green and sustainable source of liquid transportation fuels, researchers at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) have identified microbial genes that can improve both the tolerance and the production of biogasoline in engineered strains of Escherichia coli. (more…)
The first dynamic regulatory system that prevents the build-up of toxic metabolites in engineered microbes has been reported by a team of researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI). The JBEI researchers used their system to double the production in Escherichia coli (E. coli) of amorphadiene, a precursor to the premier antimalarial drug artemisinin.
Using genome-wide transcriptional analysis, the JBEI researchers identified native regions of DNA – called “promoters” – in E. coli that respond to toxic metabolites by promoting the expression of protective genes. They then developed a system based on these promoters for regulating artificial metabolic pathways engineered into the E.coli to enable the bacterium to produce amorphadiene. (more…)
Species facing widespread and rapid environmental changes can sometimes evolve quickly enough to dodge the extinction bullet. Populations of disease-causing bacteria evolve, for example, as doctors flood their “environment,” the human body, with antibiotics. Insects, animals and plants can make evolutionary adaptations in response to pesticides, heavy metals and overfishing.
Previous studies have shown that the more gradual the change, the better the chances for “evolutionary rescue” – the process of mutations occurring fast enough to allow a population to avoid extinction in changing environments. One obvious reason is that more individuals remain alive when change is gradual or moderate, meaning there are more opportunities for a winning mutation to emerge. (more…)
Berkeley Lab Researchers Develop New Tool for Making Genetic Engineering of Microbial Circuits Reliably Predictable
Synthetic biology is the latest and most advanced phase of genetic engineering, holding great promise for helping to solve some of the world’s most intractable problems, including the sustainable production of energy fuels and critical medical drugs, and the safe removal of toxic and radioactive waste from the environment. However, for synthetic biology to reach its promise, the design and construction of biological systems must be as predictable as the assembly of computer hardware.
An important step towards attaining a higher degree of predictability in synthetic biology has been taken by a group of researchers with the U.S. Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab) under the leadership of computational biologist Adam Arkin. Arkin and his team have developed an “adaptor” that makes the genetic engineering of microbial components substantially easier and more predictable by converting regulators of translation into regulators of transcription in Escherichia coli. Transcription and translation make up the two-step process by which the coded instructions of genes are used to synthesize proteins. (more…)
*Researchers coax viruses to assemble into synthetics with microstructures and properties akin to those of corneas, teeth and skin*
Using a simple, single-step process, engineers and scientists at the University of California at Berkeley recently developed a technique to direct benign, filamentous viruses called M13 phages to serve as structural building blocks for materials with a wide range of properties.
By controlling the physical environment alone, the researchers caused the viruses to self-assemble into hierarchically organized thin-film structures, with complexity that ranged from simple ridges, to wavy, chiral strands, to truly sophisticated patterns of overlapping strings of material–results that may also shed light on the self-assembly of biological tissues in nature. (more…)
*Berkeley Lab Researchers Resolve Sub-nanometer Structure of Cascade, an Ally for Human Immune System*
A complex of proteins in the bacterium E.coli that plays a critical role in defending the microbe from viruses and other invaders has been discovered to have the shape of a seahorse by researchers with the U.S Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab). This discovery holds far more implications for your own health than you might think.
In its never-ending battle to protect you from infections by bacteria, viruses, toxins and other invasive elements, your immune system has an important ally – many allies in fact. By the time you reach adulthood, some 90-percent of the cells in your body are microbial. These microbes – collectively known as the microbiome – play a critical role in preserving the health of their human host. (more…)
ANN ARBOR, Mich.—Scientists have discovered a molecular assistant called Spy that helps bacteria excel at producing proteins for medical and industrial purposes.
Bacteria are widely used to manufacture proteins used in medicine and industry, but the bugs often bungle the job. Many proteins fall apart and get cut up inside the bacteria before they can be harvested. Others collapse into useless tangles instead of folding properly, as they must in order to function normally. (more…)
