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New Insight into an Emerging Genome-Editing Tool

By Guest PostMarch 11, 2014Scienceactinomyces naeslundii, anacas9, bacteria, bacterial immune system, berkeley lab, biochemist, biophysicist, cas9, cas9 enzymes, crispr, david taylor, DNA, enzyme, eva nogales, fuguo jiang, genetic element, genetically engineered, genome editing tool, guide rna, jennifer doudna, microbes, spycas, streptococcus pyogenes

Berkeley Researchers Show Expanded Role for Guide RNA in Cas9 Interactions with DNA

The potential is there for bacteria and other microbes to be genetically engineered to perform a cornucopia of valuable goods and services, from the production of safer, more effective medicines and clean, green, sustainable fuels, to the clean-up and restoration of our air, water and land. Cells from eukaryotic organisms can also be modified for research or to fight disease. To achieve these and other worthy goals, the ability to precisely edit the instructions contained within a target’s genome is a must. A powerful new tool for genome editing and gene regulation has emerged in the form of a family of enzymes known as Cas9, which plays a critical role in the bacterial immune system. Cas9 should become an even more valuable tool with the creation of the first detailed picture of its three-dimensional shape by researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) and the University of California (UC) Berkeley. (more…)

Puzzling Question in Bacterial Immune System Answered

By Guest PostFebruary 6, 2014Sciencebacterial enzyme, bacterial immune system, bacterial microbes face, Berkeley, berkeley lab, cas9, cas9 enzyme, crispr associated, crrna molecule, eric greene, genomic dna, jennifer doudna, martin jinek, nucleic acid, pam, pam sequences, plasmids, protospacer adjacent motif, puzzling question, rna, samuel sternberg, short dna sequence, sy redding, synthetic biology, uc, university of california

Berkeley Researchers Uncover the Key to Self-Awareness in Genome Editor

A central question has been answered regarding a protein that plays an essential role in the bacterial immune system and is fast becoming a valuable tool for genetic engineering. A team of researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) and the University of California (UC) Berkeley have determined how the bacterial enzyme known as Cas9, guided by RNA, is able to identify and degrade foreign DNA during viral infections, as well as induce site-specific genetic changes in animal and plant cells. Through a combination of single-molecule imaging and bulk biochemical experiments, the research team has shown that the genome-editing ability of Cas9 is made possible by the presence of short DNA sequences known as “PAM,” for protospacer adjacent motif. (more…)

Programmable DNA Scissors Found for Bacterial Immune System

By Guest PostJuly 2, 2012Sciencearchaeon microbe, artificial enzyme, bacteria, bacterial immune system, berkeley lab, cas9 protein, crispr, crispr loci, crrna guide sequence, crrna molecule, dna scissors, double rna structure, eukaryotic organism, foreign dna, fungi, genetic element, genetic engineers, genomics researchers, hhmi, human cell, ines fonfara, jennifer doudna, krzysztof chylinski, martin jinek, michael hauer, tracrrna

Discovery Could Lead to Editing Tool for Genomes

Genetic engineers and genomics researchers should welcome the news from the Lawrence Berkeley National Laboratory (Berkeley Lab) where an international team of scientists has discovered a new and possibly more effective means of editing genomes. This discovery holds potentially big implications for advanced biofuels and therapeutic drugs, as genetically modified microorganisms, such as bacteria and fungi, are expected to play a key role in the green chemistry production of these and other valuable chemical products.

Jennifer Doudna, a biochemist with Berkeley Lab’s Physical Biosciences Division and professor at the University of California (UC) Berkeley, helped lead the team that identified a double-RNA structure responsible for directing a bacterial protein to cleave foreign DNA at specific nucleotide sequences. Furthermore, the research team found that it is possible to program the protein with a single RNA to enable cleavage of essentially any DNA sequence. (more…)

Close Up Look at a Microbial Vaccination Program

By Guest PostOctober 3, 2011Health, Sciencebacterium, berkeley lab, blake wiedenheft, casa, casb, case, cells, clustered regularly interspaced short palindromic repea, complex of proteins, crispr, cryo electron microscopy, e coli, escherichia coli, eva nogales, gabriel lander, human pathogens, immune system, infections by bacteria, invading pathogens, jennifer doudna, john van der oost, kaihong zhou, microbe, microbial, microbial immune systems, microbiome, perturbations, protein complex called cascade, seahorse, short interfering rnas, sirnas, symbioses, toxins, unit of dna, viruses

*Berkeley Lab Researchers Resolve Sub-nanometer Structure of Cascade, an Ally for Human Immune System*

A complex of proteins in the bacterium E.coli that plays a critical role in defending the microbe from viruses and other invaders has been discovered to have the shape of a seahorse by researchers with the U.S Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab). This discovery holds far more implications for your own health than you might think.

In its never-ending battle to protect you from infections by bacteria, viruses, toxins and other invasive elements, your immune system has an important ally – many allies in fact. By the time you reach adulthood, some 90-percent of the cells in your body are microbial. These microbes – collectively known as the microbiome – play a critical role in preserving the health of their human host. (more…)

Scientists identify key enzyme in microbial immune system

By Guest PostSeptember 10, 20102 commentsSciencearchaea, bacteria, berkeley lab, Bill and Melinda Gates foundation, clustered regularly interspaced short palindromic repea, crispr, crrna molecule, csy4 enzyme, DNA, doudna group, genetic element, jennifer doudna, microbes, movie, nucleic acid, nucleic acid-based immune system, plasmids, rachel haurwitz, terminator

Imagine a war in which you are vastly outnumbered by an enemy that is utterly relentless – attacking you is all it does. The intro to another Terminator movie? No, just another day for microbes such as bacteria and archaea, which face a never-ending onslaught from viruses and invading strands of nucleic acid known as plasmids.

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Tag Archives: jennifer doudna

New Insight into an Emerging Genome-Editing Tool

Puzzling Question in Bacterial Immune System Answered

Programmable DNA Scissors Found for Bacterial Immune System

Close Up Look at a Microbial Vaccination Program

Scientists identify key enzyme in microbial immune system