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Brighter, Smaller Probes to Uncover the Secret Lives of Proteins

By Guest PostMay 10, 20121 commentSciencealexis ostrowski, andrew mueller, berkeley lab, bruce cohen, cellular mechanism, daniel gargas, delia milliron, disease, elan katz, emory chan, erbium, function, gang han, imagine tracking, james schuck, lanthanide, live cell, los angeles, molecule, nanocrystal, nanoparticle, nayf4, photobleach, protein, radio transmitter, sodium yttrium fluoride, vistamar school, wanda robot, ytterbium

Berkeley Lab scientists create nanoparticle probes that may lead to a better understanding of diseases

Imagine tracking a deer through a forest by clipping a radio transmitter to its ear and monitoring the deer’s location remotely. Now imagine that transmitter is the size of a house, and you understand the problem researchers may encounter when they try to use nanoparticles to track proteins in live cells.

Understanding how a protein moves around a cell helps researchers understand the protein’s function and the cellular mechanisms for making and processing proteins. This information also helps researchers study disease, which at a cellular level may mean that a protein is malfunctioning, stops being made, or is sent to the wrong part of the cell. But nanoparticle probes that are too big can disrupt a protein’s normal activities. (more…)

Molecular Spectroscopy Tracks Living Mammalian Cells in Real Time as They Differentiate

By Guest PostMay 2, 2012Scienceals, alzheimer's disease, amino acid residues, apoptosis, bacteria, berkeley lab, carbohydrates, cell change, cell death, children, diabetes, fibroblasts, fluorescent dyes, fourier transform infrared, ftir, function, hans bechtel, hoi ying, hyperspectromicroscopy, ir, kevin loutherback, liang chen, lipids, macular degeneration, michael martin, mitochondrial diseases, pc12, phosphorylation, phosphorylation process, power plant, protein, rafael gomez sjoberg, retina, san diego, serine, spectromicroscopy, threonine, tyrosine, ultraviolet, university of california, zhao hao

Berkeley Lab scientists demonstrate the promise of synchrotron infrared spectroscopy of living cells for medical applications

Knowing how a living cell works means knowing how the chemistry inside the cell changes as the functions of the cell change. Protein phosphorylation, for example, controls everything from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death (apoptosis), in cells from bacteria to humans. It’s a chemical process that has long been intensively studied, not least in hopes of treating or eliminating a wide range of diseases. But until now the close-up view – watching phosphorylation work at the molecular level as individual cells change over time – has been impossible without damaging the cells or interfering with the very processes that are being examined.

“To look into phosphorylation, researchers have labeled specific phosphorylated proteins with antibodies that carry fluorescent dyes,” says Hoi-Ying Holman of the U.S. Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab). “That gives you a great image, but you have to know exactly what to label before you can even begin.” (more…)

Locked RNA Editing Yields Odd Fly Behavior

By Guest PostApril 26, 2012Scienceabiotic factor, als, arthur sugden, asli sahin, brown university, cell, charles lawrence, dadar, enzyme adar, epilepsy, factor, fruit fly, function, genetic, jacquelyn dorsky, james jepson, lauren alpert, locking down, lou gehrigs disease, nucleus, organism, phenomenon, protein, rna, robert reenan, suicidal depression, temperature, wallflowers, yiannis savva

At the level of proteins, organisms can adapt by editing their RNA — and an editor can even edit itself. Brown University scientists working with fruit flies found that “locking down” the self-editing process at two extremes created some strange behaviors. They also found that the process is significantly affected by temperature.

PROVIDENCE, R.I. [Brown University] — Because a function of RNA is to be translated as the genetic instructions for the protein-making machinery of cells, RNA editing is the body’s way of fine-tuning the proteins it produces, allowing us to adapt. The enzyme ADAR, which does this editing job in the nervous system of creatures ranging from mice to men, even edits itself. In a new study that examined the self-editing process and locked it down at two extremes in fruit flies, Brown University scientists found some surprising insights into how this “fine-tuning of the fine-tuner” happens, including bizarre behavioral effects that come about when the self-editor can’t edit. (more…)

Rebuilding a Heart, Saving a Life

By Guest PostApril 19, 2012Healthamerican patient, angela irizarry, birthday, blood vessel, blue babies, bone marrow, bridgeport, cardiac surgeon, christopher breuer, circulatory system, claudia irizarry, congenital heart defect, connecticut, fda, function, gary kopf, hypoplastic, infection, inflammatory response, left heart syndrome, mark saltzman, natural cell, operation, organ, oxygenated blood, polymer, synthetic grafts, synthetic nature, tissue engineered, toshi shinoka, united states, yale doctor, young girl

A young girl in Bridgeport, Connecticut, born with one of the most serious, life-threatening congenital heart defects known, is on her way to living a normal life thanks to Yale doctors who developed and performed the first operation of its kind in the United States.

Angela Irizarry was the first American patient to receive a tissue-engineered blood vessel made of her own natural cells. (more…)

Scientists Find Possible Cause of Movement Defects in Spinal Muscular Atrophy

By Guest PostApril 15, 2012Healthamino acid, childhood disorder, christine beattie, emily caldwell, function, gene, genetic link, genetic manipulation, le hao, levels of protein, marc wolman, michael granato, motor neuron, muscle weakness, nerve cell, plastin 3, protein, sma, smn, spinal cord, spinal muscular atrophy, zebrafish

COLUMBUS, Ohio – An abnormally low level of a protein in certain nerve cells is linked to movement problems that characterize the deadly childhood disorder spinal muscular atrophy, new research in animals suggests.

Spinal muscular atrophy, or SMA, is caused when a child’s motor neurons – nerve cells that send signals from the spinal cord to muscles – produce insufficient amounts of what is called survival motor neuron protein, or SMN. This causes motor neurons to die, leading to muscle weakness and the inability to move. (more…)

Many Young People Would Rather Surf the Web than Drive a Car

By Guest PostApril 5, 2012General, Technologyage, bernie degroat, brandon schoettle, canada, driver, environmental consequences, finland, function, Germany, great britain, information highway, internet users, Israel, Japan, latvia, license, michael sivak, norway, people, poland, societal wealth, south korea, spain, Sweden, switzerland, teenager, the netherlands, traffic injury, travel, united states, university of michigan

ANN ARBOR, Mich.— More young adults today would rather hit the information highway than the open highway, say University of Michigan researchers.

In a new study in the journal Traffic Injury Prevention, Michael Sivak and Brandon Schoettle of the U-M Transportation Research Institute found that having a higher proportion of Internet users was associated with lower licensure rates among young persons.

And this is just not in the United States; it’s happening in other countries, too. (more…)

Loss of Appetite Deciphered in Brain Cell Circuit

By Guest PostMarch 14, 2012Scienceanorexia nervosa, body, brain, brain development, cancer chemotherapy, excitatory signal, experimental result, food aversions, function, gaba, glutamate, hypothalamus, loss of appetite, meal, mice, michael s clark, michael schwartz, nausea, nerve cells, nucleus tractus solitarus, parabrachial nucleus, point of emaciation, qi wu, ralph brinster, receptor, richard d palmiter, starvation, substance, transgenic mice, university of pennsylvania, university of washington

The meal is pushed way, untouched. Loss of appetite can be a fleeting queasiness or continue to the point of emaciation. While it’s felt in the gut, more is going on inside the head.

New findings are emerging about brain and body messaging pathways that lead to loss of appetite, and the systems in place to avoid starvation. (more…)

It Takes Two to Tango: Pairs of Entwined Proteins Handle The Stress

By Guest PostMarch 2, 2012Sciencebacterial protein, bleach, cell paper, dana reichmann, dance floor, function, helper protein, hsp33, jim erickson, michael fitzgerald, molecular chaperone, oxidants, protein, protein folding process, stress specific, tango, university of michigan, ursula jakob, ying xu

ANN ARBOR, Mich.— Imagine two tango dancers sweeping across the dance floor and suddenly encountering a slick spot. To avoid a slip or even a nasty tumble, the pair must work together to support one another and glide safely through the stressful moment.

In a similar way, a certain type of helper protein called a stress-specific molecular chaperone prevents its client proteins from collapsing during stressful situations by uncoiling a long, supportive arm that wraps around them, a University of Michigan-led research team has shown. (more…)

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