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Berkeley Lab Researchers Unveil Technique for Easy Comparisons of Proteins in Solution

A revolutionary X-ray analytical technique that enables researchers at a glance to identify structural similarities and differences between multiple proteins under a variety of conditions has been developed by researchers with the U.S. Department of Energy (DOE)’s Lawrence Berkeley National Laboratory (Berkeley Lab). As a demonstration, the researchers used this technique to gain valuable new insight into a protein that is a prime target for cancer chemotherapy.

“Proteins and other biological macromolecules are moving machines whose power is often derived from how their structural conformations change in response to their environment,” says Greg Hura, a scientist with Berkeley Lab’s Physical Biosciences Division. “Knowing what makes a protein change has incredible value, much like knowing that stepping on a gas pedal makes the wheels of a car spin.” (more…)

Bold Move Forward in Molecular Analyses

By Guest PostMay 21, 2013Scienceabsolute scale, berkeley lab, cell biology, concentration, control, data collection, dataset, john tainer, macromolecules, molecular analyses, molecular machines, nanoparticle, neutrons, protein, robert rambo, sans, sas, sas analysis, sas based statistic, saxs, small angle scattering, solution, structural state, volume of correlation

Berkeley Lab Researchers Develop New Metrics for X-ray and Neutron Analysis of Flexible Macromolecules

A dramatic leap forward in the ability of scientists to study the structural states of macromolecules such as proteins and nanoparticles in solution has been achieved by a pair of researchers with the U.S. Department of Energy (DOE)’s Lawrence Berkeley National Laboratory (Berkeley Lab). The researchers have developed a new set of metrics for analyzing data acquired via small angle scattering (SAS) experiments with X-rays (SAXS) or neutrons (SANS). Among other advantages, this will reduce the time required to collect data by up to 20 times.

“SAS is the only technique that provides a complete snapshot of the thermodynamic state of macromolecules in a single image,” says Robert Rambo, a scientist with Berkeley Lab’s Physical Biosciences Division, who developed the new SAS metrics along with John Tainer of Berkeley Lab’s Life Sciences Division and the Scripps Research Institute. (more…)

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Comparing Proteins at a Glance

Bold Move Forward in Molecular Analyses